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Updated: Apr 19, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
Hydroxamic acids block replication of hepatitis C virus
1Center for Drug Design, Academic Health Center, University of Minnesota , 516 Delaware Street S.E., Minneapolis, Minnesota 55455, United States.
Abstract:
Intrigued by the role of protein acetylation in hepatitis C virus (HCV) replication, we tested known histone deacetylase (HDAC) inhibitors and a focused library of structurally simple hydroxamic acids for inhibition of a HCV subgenomic replicon. While known HDAC inhibitors with varied inhibitory profiles proved to be either relatively toxic or ineffective, structure-activity relationship (SAR) studies on cinnamic hydroxamic acid and benzo[b]thiophen-2-hydroxamic acid gave rise to compounds 22 and 53, which showed potent and selective anti-HCV activity and therefore are promising starting points for further structural optimization and mechanistic studies.
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