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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Apr 19, 2026

Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation

Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation

Published on: November 3, 2018

Translating the MDS flow cytometric score into clinical practice.

Michiel Heron¹, Elisabeth Dovern, Liesbeth E Bakker-Jonges

¹Medical Laboratories, Department of Immunology, Reinier de Graaf Groep, Delft, The Netherlands.

Cytometry. Part B, Clinical Cytometry

|December 11, 2014

Summary

This study validates a flow cytometric scoring (FCM-score) method for diagnosing myelodysplastic syndromes (MDS). The FCM-score accurately distinguishes low-grade MDS from other cytopenias, confirming its clinical utility.

Keywords:

flow cytometry immunophenotype myelodysplastic syndrome

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Last Updated: Apr 19, 2026

Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation

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Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

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An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry

Published on: May 13, 2019

Area of Science:

Hematology
Oncology
Clinical Pathology

Background:

Myelodysplastic syndromes (MDS) are myeloid neoplasms characterized by cytopenia and dysplasia.
Accurate diagnosis of low-grade MDS is crucial for appropriate patient management.
Existing diagnostic methods may require refinement for improved specificity.

Purpose of the Study:

To evaluate the applicability and diagnostic performance of a published flow cytometric scoring (FCM-score) method in a Dutch patient cohort.
To assess the FCM-score's ability to differentiate low-grade MDS from non-clonal cytopenias.
To explore alternative immunophenotypic markers for improved blast identification within the FCM-score framework.

Main Methods:

Retrospective analysis of patient data from a large peripheral teaching hospital.
Application of the established FCM-score algorithm to identify MDS.
Utilized CD10 and CD19 expression on progenitor B-cell blasts for improved discrimination within the CD34+/low CD45+ population.

Main Results:

The FCM-score demonstrated high sensitivity and specificity in diagnosing low-grade MDS.
Clinically significant positive and negative likelihood ratios were observed, supporting the score's diagnostic value.
CD10/CD19 positivity offered a precise and potentially more convenient method for distinguishing progenitor B-cell blasts from myeloid blasts.

Conclusions:

The FCM-score is a valuable and validated tool for diagnosing low-grade MDS in the studied patient population.
The findings support the integration of the FCM-score into routine clinical practice for MDS evaluation.
Refined blast identification strategies enhance the robustness of flow cytometry in MDS diagnosis.