Modification of shell vial centrifugation method for detection of herpes simplex virus

Area of Science:

• Virology
• Clinical Diagnostics
• Microbiology

Background:

• The shell vial centrifugation method is a standard technique for detecting herpes simplex virus (HSV).
• Optimizing diagnostic procedures can improve efficiency and accuracy in clinical laboratories.

Purpose of the Study:

• To evaluate a modified shell vial centrifugation method for herpes simplex virus detection.
• To assess the impact of adding maintenance medium before specimen inoculation on diagnostic performance.

Main Methods:

• The shell vial centrifugation method was adapted by introducing maintenance medium prior to specimen inoculation.
• The modified method's performance was compared against the conventional post-centrifugation medium addition technique.

Main Results:

• The modified shell vial centrifugation method demonstrated sensitivity and specificity values comparable to the traditional method.
• No significant difference in diagnostic accuracy was observed between pre- and post-inoculation medium addition.

Conclusions:

• The modified shell vial centrifugation method, with pre-inoculation medium addition, is a viable alternative for herpes simplex virus detection.
• This modification offers a potentially streamlined workflow without compromising diagnostic performance.