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Updated: Apr 19, 2026

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
Published on: May 22, 2017
Mucolipidosis type IV protein TRPML1-dependent lysosome formation
Austin Miller1, Jessica Schafer, Cameron Upchurch
1Department of Molecular and Cellular Biology, Life Sciences South Room 531, University of Arizona, Tucson, AZ, 85721, USA.
Abstract:
Lysosomes are dynamic organelles that undergo cycles of fusion and fission with themselves and with other organelles. Following fusion with late endosomes to form hybrid organelles, lysosomes are reformed as discrete organelles. This lysosome reformation or formation is a poorly understood process that has not been systematically analyzed and that lacks known regulators. In this study, we quantitatively define the multiple steps of lysosome formation and identify the first regulator of this process.
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