Peptide-mediated delivery: an overview of pathways for efficient internalization

Area of Science:

• Biochemistry
• Molecular Biology
• Drug Delivery

Background:

• Cellular delivery and bioavailability challenges hinder novel cancer and gene therapies.
• Cell-penetrating peptides (CPPs) offer solutions for intracellular delivery of therapeutic cargos.
• CPPs have shown promise in experimental therapies, including muscular dystrophies.

Purpose of the Study:

• To review the mechanisms of CPP-mediated cellular entry and cargo delivery.
• To highlight advances in enhancing CPP delivery efficacy and endosomal escape.
• To analyze CPP delivery of oligonucleotides and proteins.

Main Methods:

• Literature review of cell-penetrating peptide mechanisms and applications.
• Analysis of recent advancements in delivery efficacy and endosomal escape strategies.
• Examination of CPP-mediated delivery of oligonucleotides and proteins.

Main Results:

• CPPs facilitate intracellular access for therapeutic molecules.
• Advances improve CPP efficacy and promote cargo release from endosomes.
• Oligonucleotide and protein delivery via CPPs are analyzed, with expanded tools for targeted delivery.

Conclusions:

• CPPs are crucial for overcoming cellular delivery barriers in modern therapeutics.
• Enhanced CPP strategies improve the potential of gene and cancer therapies.
• Targeted delivery of oligonucleotides and proteins is increasingly feasible using CPPs.