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Mu transposable elements are structurally diverse and distributed throughout the genus Zea.

L E Talbert1, G I Patterson, V L Chandler

  • 1Institute of Molecular Biology, University of Oregon, Eugene 97403.

Journal of Molecular Evolution
|July 1, 1989
PubMed
Summary

Maize Mutator stocks have high mutation rates from transposable Mu elements. New elements, Mu4 and Mu5, found in non-Mutator maize, share Mu terminal repeats but have unique internal sequences, suggesting ancient transposition activity.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Plant Science

Background:

  • Maize Mutator stocks exhibit high mutation rates driven by Mu transposable elements.
  • Non-Mutator maize stocks show lower mutation rates but contain sequences homologous to Mu terminal inverted repeats.
  • These homologous sequences often lack similarity to the internal regions of previously cloned Mu elements.

Purpose of the Study:

  • To clone and characterize novel Mu-related sequences in non-Mutator maize.
  • To investigate the structural organization and evolutionary origins of these elements.
  • To explore the potential for independent transposition of Mu termini.

Main Methods:

  • DNA hybridization techniques to identify Mu-related sequences in non-Mutator maize (line B37).

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  • Cloning and sequencing of identified homologous DNA fragments (Mu4 and Mu5).
  • Comparative sequence analysis of Mu4, Mu5, and known Mu elements.

    • Main Results:

    • Two new transposable elements, Mu4 and Mu5, were cloned from non-Mutator maize.
    • Mu4 and Mu5 possess characteristic Mu terminal inverted repeats (TIRs) but unrelated internal sequences.
    • Both elements are flanked by 9-bp direct repeats and exhibit unusually long TIRs extending internally.
    • Distribution analysis suggests Mu elements are ancient components of the maize genome.

    Conclusions:

    • Mu4 and Mu5 represent novel Mu family members with distinct internal sequences.
    • The conserved structure of Mu termini, despite variable internal DNA, suggests potential for independent transposition.
    • Mu elements are likely ancient and have shaped the maize genome over evolutionary time.