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Promotion of Survival and Differentiation of Neural Stem Cells with Fibrin and Growth Factor Cocktails after Severe Spinal Cord Injury
Published on: July 27, 2014
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Fibronectin Matrix Assembly after Spinal Cord Injury
Yunjiao Zhu1, Cynthia Soderblom1, Michelle Trojanowsky1
1Department of Neurological Surgery, Miami Project to Cure Paralysis, University of Miami School of Medicine , Miami, Florida.
Journal of Neurotrauma
|December 11, 2014
Summary
Fibroblasts, not myeloid cells, are the primary source of fibronectin after spinal cord injury (SCI). This fibronectin assembles into an inhibitory matrix, hindering axon regeneration.
Area of Science:
- Neuroscience
- Regenerative Medicine
- Cell Biology
Background:
- Spinal cord injury (SCI) leads to fibrotic scar formation.
- This scar contains perivascular fibroblasts and fibronectin, inhibiting axon regeneration.
Purpose of the Study:
- To investigate the source of fibronectin in the SCI fibrotic scar.
- To understand the mechanism of fibronectin matrix assembly after SCI.
Main Methods:
- Fibronectin deletion in myeloid cells in a rat SCI model.
- Analysis of fibronectin presence and matrix assembly at 7 days post-SCI.
Main Results:
- Fibroblasts, not myeloid cells, are the major source of fibronectin in the SCI scar.
- Fibronectin is initially soluble and assembles into a matrix by 7 days post-SCI.
- Integrin α5β1 on macrophages/microglia may mediate fibronectin matrix assembly.
Conclusions:
- Fibroblast-derived fibronectin contributes to the inhibitory SCI scar.
- Understanding fibronectin matrix assembly offers therapeutic targets for promoting axon regeneration after SCI.
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