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Cardiac Stress Test Induced by Dobutamine and Monitored by Cardiac Catheterization in Mice
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Cardiac Stress Test Induced by Dobutamine and Monitored by Cardiac Catheterization in Mice

Published on: February 10, 2013

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Inhibitory effects of dobutamine on human gastric adenocarcinoma

Hui-Xia Zheng1, Li-Na Wu1, Hong Xiao1

  • 1Hui-Xia Zheng, Li-Na Wu, Hong Xiao, Qian Du, Jian-Fang Liang, Department of Pathology, The First Hospital, Shanxi Medical University, Taiyuan 030024, Shanxi Province, China.

World Journal of Gastroenterology
|December 11, 2014
PubMed
Abstract

Insights

Dobutamine effectively inhibits gastric adenocarcinoma cell growth, migration, and invasion. This drug also induces apoptosis and cell cycle arrest, suggesting potential as a neoadjuvant therapy for gastric cancer.

Area of Science:

  • Oncology
  • Cancer Biology
  • Pharmacology

Background:

  • Gastric adenocarcinoma is a significant global health concern.
  • Exploring novel therapeutic agents for gastric cancer is crucial.

Purpose of the Study:

  • To investigate the inhibitory effects of dobutamine on gastric adenocarcinoma cells.
  • To evaluate dobutamine's impact on cell viability, proliferation, migration, invasion, cell cycle, and apoptosis.

Main Methods:

  • Utilized gastric adenocarcinoma cells (SGC-7901) treated with dobutamine.
  • Assessed cell viability (MTT assay), migration (wound healing), proliferation (colony formation), invasion (Transwell assay).
  • Analyzed cell cycle and apoptosis (flow cytometry), and Yes-associated protein (YAP) expression (Western blot, immunocytochemistry).

Main Results:

  • Dobutamine significantly inhibited cell growth, migration, colony formation, and invasion.
  • Dobutamine induced G1/S phase cell cycle arrest and increased apoptosis.
  • Dobutamine treatment altered YAP expression, with reduced phosphorylated YAP in the cytosol.

Conclusions:

  • Dobutamine exhibits significant inhibitory effects on gastric adenocarcinoma cells.
  • Dobutamine shows promise as a neoadjuvant therapy for gastric cancer and potentially other tumors.
Keywords:
DobutamineGastric adenocarcinoma cellsHippo pathwayHuman gastric adenocarcinoma cell line SGC-7901TherapyYes-associated protein

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