Decrease of free radical concentrations in humans following consumption of a high antioxidant capacity natural...

Area of Science:

• Biomedical Science
• Nutritional Science
• Analytical Chemistry

Background:

• In vitro antioxidant assays like ORAC are limited for in vivo applications.
• Understanding diet's impact on reactive oxidant species (ROS) requires direct in vivo measurement.
• Dihydrorhodamine-6G (DHR6G) offers a non-specific fluorescent probe for total ROS quantification.

Purpose of the Study:

• To investigate the in vivo quantification of total ROS in human subjects using DHR6G.
• To assess the efficacy of an antioxidant-rich fruit, vegetable, and herb blend (Spectra™) in mitigating ROS.
• To establish a reliable and efficient method for evaluating antioxidant effectiveness against ROS in vivo.

Main Methods:

• Twelve human participants consumed 100 mg of Spectra™.
• Blood samples were collected at 0, 60, 120, and 180 minutes post-ingestion.
• Serum ROS concentrations were quantified using the DHR6G fluorescent probe.

Main Results:

• The DHR6G method successfully quantified ROS levels in human serum.
• Eleven out of twelve participants exhibited a significant decrease in ROS concentrations.
• This indicates a positive response to the antioxidant blend intake.

Conclusions:

• The DHR6G assay provides a reliable and efficient in vivo method for measuring total ROS.
• Antioxidant-rich supplements, like Spectra™, can effectively reduce in vivo ROS levels in humans.
• This approach advances the study of dietary impacts on oxidative stress.