Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

10.3K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
10.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Real-World Insights on Switching Aflibercept Dosage for Enhanced Outcomes in Retinal Vein Occlusion (RISE-RVO): visual, anatomical and durability outcomes.

The British journal of ophthalmology·2026
Same author

Orthokeratology and retinal risk: a new dimension in myopia control.

Translational pediatrics·2026
Same author

The diagnostic accuracy of AI-assisted diabetic retinopathy screening in primary care: a prospective validation study.

Scandinavian journal of primary health care·2026
Same author

Real-world insights on switching aflibercept dosage for enhanced outcomes in age-related macular degeneration (RISE-AMD): Safety data.

Acta ophthalmologica·2026
Same author

Real-World Outcomes after Switch to Aflibercept 8 mg in Neovascular AMD: Twelve Months Follow-up on 654 Eyes.

Retina (Philadelphia, Pa.)·2026
Same author

Characteristics of Uveitis in European Tertiary Ophthalmology Centers: A Systematic Review and Meta-Analysis.

Ocular immunology and inflammation·2026

Related Experiment Video

Updated: Apr 19, 2026

Dissection of Human Vitreous Body Elements for Proteomic Analysis
05:05

Dissection of Human Vitreous Body Elements for Proteomic Analysis

Published on: January 23, 2011

32.8K

Analytical platforms in vitreoretinal proteomics.

Lasse Jørgensen Cehofski1, Nakul Mandal, Bent Honoré

  • 1Department of Ophthalmology, Aalborg University Hospital, Hobrovej 18-22, 9100, Aalborg; Denmark.

Bioanalysis
|December 16, 2014
PubMed
Summary
This summary is machine-generated.

Proteomic technologies offer powerful insights into vitreous and retinal proteins in health and disease. Careful sample preparation and multi-technique analysis are crucial for accurate results in proteomic studies.

More Related Videos

Dissection of Human Retina and RPE-Choroid for Proteomic Analysis
06:54

Dissection of Human Retina and RPE-Choroid for Proteomic Analysis

Published on: November 12, 2017

12.2K
Sample Preparation for Mass-spectrometry-based Proteomics Analysis of Ocular Microvessels
11:32

Sample Preparation for Mass-spectrometry-based Proteomics Analysis of Ocular Microvessels

Published on: February 22, 2019

12.0K

Related Experiment Videos

Last Updated: Apr 19, 2026

Dissection of Human Vitreous Body Elements for Proteomic Analysis
05:05

Dissection of Human Vitreous Body Elements for Proteomic Analysis

Published on: January 23, 2011

32.8K
Dissection of Human Retina and RPE-Choroid for Proteomic Analysis
06:54

Dissection of Human Retina and RPE-Choroid for Proteomic Analysis

Published on: November 12, 2017

12.2K
Sample Preparation for Mass-spectrometry-based Proteomics Analysis of Ocular Microvessels
11:32

Sample Preparation for Mass-spectrometry-based Proteomics Analysis of Ocular Microvessels

Published on: February 22, 2019

12.0K

Area of Science:

  • Ophthalmology
  • Proteomics
  • Biochemistry

Background:

  • The vitreous body and retina are complex biological tissues crucial for vision.
  • Understanding their protein composition is vital for diagnosing and treating ocular diseases.
  • Proteomic technologies provide powerful tools for this investigation.

Purpose of the Study:

  • To review key considerations for applying proteomic technologies to the vitreous and retina.
  • To highlight the importance of sample processing, analytical platforms, and bioinformatics.
  • To discuss quantitative proteomics strategies for ocular tissues.

Main Methods:

  • Review of current proteomic technologies and methodologies.
  • Discussion of sample preparation techniques for vitreous and retinal tissues.
  • Comparison of label-based and label-free quantitative proteomics approaches.

Main Results:

  • Appropriate sample handling, analytical platforms, and bioinformatics are essential for reliable proteomic data.
  • Potential interfering proteins require careful management during sample preparation and data analysis.
  • Employing multiple quantification techniques and database search programs enhances proteome coverage and result robustness.

Conclusions:

  • Proteomics is a valuable tool for studying vitreous and retinal proteins in normal and pathological conditions.
  • Methodological rigor in sample processing and data analysis is paramount for meaningful insights.
  • Integrated approaches using diverse quantitative techniques yield more comprehensive and dependable proteomic findings.