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Updated: Apr 19, 2026
Continuously-stirred Anaerobic Digester to Convert Organic Wastes into Biogas: System Setup and Basic Operation
Published on: July 13, 2012
The effects of initial substrate concentration, C/N ratio, and temperature on solid-state anaerobic digestion from
Zhiying Yan1, Zilin Song1, Dong Li1
1Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Science, Chengdu 610041, China.
Abstract:
This study investigated the possibilities of improving the biogasification from solid-state anaerobic digestion (SS-AD) of composting rice straw (RS) based on the optimized digestion temperature, initial substrate concentration (ISC) and C/N ratio. RS compounds, such as lignin, cellulose, and hemicellulose, were significantly degraded after composting. A significant interactive effect of temperature, ISC and C/N ratio was found on the biogasification of SS-AD of composting RS, and a maximum biogas production was achieved at 35.6°C, with a 20% ISC and a C/N ratio of 29.6:1. The verification experiment confirmed the optimization results. High-throughput sequencing analysis indicated that microbial communities in the SS-AD mainly consist of Methanobacteria, Bacteroidia, Clostridia, Betaproteobacteria, and Gammaproteobacteria. A dominant Methanobacteria was shifted from Methanobacterium to Methanoculleus during the SS-AD process. This study provides novel information about the interdependent effects and microbial behavior of AD.
