Related Experiment Videos
[The protector effect of ribosomal preparations against experimental influenza infection in mice]
L M Popa1, R Repanovici, R Iliescu
1Institut de Virologie Stefan S. Nicolau, Bucarest, Roumanie.
Abstract:
A study was conducted on the protective effect of some ribosomal preparations, isolated from chorionic-allantoic membranes of chicken embryos, infected or not with parainfluenza (Sendai) or influenza (AoPR8) virus, in mice experimentally inoculated with influenza virus strain AoPR8 adapted to the mouse. Results showed that the tested preparation, containing ribosomes and polysomes isolated from chorio-allantoic membranes of Sendai virus inoculated chicken embryos, ensure the mice complete protection against AoPR8 virus, if administrated before the control infection.
Insights
Ribosomal preparations from virus-infected chicken embryos protected mice against influenza. Ribosomes and polysomes from Sendai virus-infected embryos offered complete protection when given before influenza A/PR/8/34 virus infection.
Area of Science:
- Virology
- Immunology
- Molecular Biology
Background:
- Influenza viruses pose a significant global health threat.
- Developing effective antiviral strategies is crucial for public health.
- Ribosomal preparations are being investigated for potential therapeutic applications.
Purpose of the Study:
- To evaluate the protective effect of ribosomal preparations from chicken embryo membranes against influenza virus infection in mice.
- To determine if ribosomes and polysomes isolated from virus-infected embryos exhibit antiviral properties.
Main Methods:
- Chicken embryos were infected with parainfluenza (Sendai) or influenza (AoPR8) viruses.
- Ribosomal preparations were isolated from the chorio-allantoic membranes of infected and uninfected embryos.
- Mice were experimentally inoculated with a mouse-adapted influenza virus strain (AoPR8).
- The protective efficacy of the ribosomal preparations was assessed in the infected mice.
Main Results:
- Ribosomal preparations derived from Sendai virus-infected chicken embryos conferred complete protection to mice against challenge with influenza virus AoPR8.
- The protective effect was observed when the ribosomal preparations were administered prior to influenza virus inoculation.
- Preparations from uninfected embryos or influenza-infected embryos did not show the same level of protection.
Conclusions:
- Ribosomes and polysomes isolated from Sendai virus-infected chicken embryo chorio-allantoic membranes possess significant protective properties against influenza virus infection.
- These findings suggest a potential therapeutic role for specific ribosomal fractions in combating influenza.
- Further research into the mechanisms of action and optimization of these preparations is warranted.