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Technical note: a pilot study using a mouse mastitis model to study differences between bovine associated

K Breyne1, S De Vliegher2, A De Visscher2

  • 1Department of Pharmacology, Toxicology and Biochemistry, University of Ghent, Salisburylaan 133, 9820 Merelbeke, Belgium.

Journal of Dairy Science
|December 16, 2014
PubMed
Summary
This summary is machine-generated.

A mouse mastitis model effectively mimics bovine Coagulase-negative staphylococci (CNS) infections. Different CNS species and strains elicit varying immune responses in mice, highlighting their distinct pathogenic potential.

Keywords:
bacterial growthcoagulase-negative staphylococci strainmouse mastitis modelneutrophil influxproinflammatory cytokine

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Area of Science:

  • Veterinary Microbiology
  • Immunology
  • Infectious Diseases

Background:

  • Coagulase-negative staphylococci (CNS) are recognized as minor mastitis pathogens or commensal microbiota in cattle.
  • Emerging evidence points to significant epidemiological and genetic variations within the diverse CNS group.
  • Understanding these differences is crucial for developing targeted mastitis control strategies.

Purpose of the Study:

  • To evaluate a mouse mastitis model for its suitability in differentiating responses to various CNS species and strains.
  • To compare the host immune response induced by different CNS strains against Staphylococcus aureus.
  • To investigate the differential inflammatory and cytokine profiles elicited by epidemiologically distinct CNS isolates.

Main Methods:

  • A mouse model was employed, comparing Staphylococcus aureus (positive control) with multiple CNS strains (Staphylococcus chromogenes, Staphylococcus fleurettii).
  • Experiments included dose titration and comparative analysis of host response at 24, 28, and 48 hours post-inoculation.
  • Bacterial load, neutrophil influx, and proinflammatory cytokine (IL-6, IL-1β, TNF-α) levels were quantified.

Main Results:

  • The mouse model successfully mimicked bovine CNS mastitis, with CNS strains inducing lower bacterial growth and less pronounced inflammation than S. aureus.
  • Staphylococcus fleurettii showed the lowest bacterial growth among CNS strains, while a S. chromogenes strain from a teat apex induced a variable IL-1β response.
  • CNS inoculation resulted in localized IL-1β induction, with S. aureus additionally inducing IL-6, but not TNF-α.

Conclusions:

  • The intraductal mouse model is a viable tool for studying bovine CNS mastitis and differentiating CNS species/strains.
  • Epidemiologically distinct CNS isolates induce differential innate immune responses in the murine mammary gland.
  • This model holds potential for future research into CNS pathogenesis and host-pathogen interactions.