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Updated: Apr 19, 2026

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Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques
Published on: May 2, 2017
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Two-handed assisted laparoscopic surgery: Evaluation in an animal model
Eduardo Sanchez-de-Badajoz1, Pilar Sanchez-Gallegos1, Jose Manuel Martinez-Moreno1
1Department of Surgery, School of Medicine, Malaga, Spain.
Summary
This study demonstrates the feasibility of two-handed laparoscopy for retroperitoneal lymph node dissection in pigs. This novel approach simplifies procedures and reduces surgery time compared to traditional open surgery.
Area of Science:
- Minimally Invasive Surgery
- Surgical Innovation
- Laparoscopic Techniques
Background:
- Traditional open surgery often requires extensive incisions.
- Laparoscopic surgery aims to reduce invasiveness but can be limited by instrument manipulation.
- A novel approach using two hands laparoscopically could enhance surgical capabilities.
Purpose of the Study:
- To assess the feasibility of a new two-handed laparoscopic technique.
- To evaluate this method in a porcine model for retroperitoneal lymph node dissection (L-RPLND).
- To compare the safety and efficiency with open surgical procedures.
Main Methods:
- A porcine model was used for the retroperitoneal lymph node dissection.
- Two abdominal incisions were made to introduce both hands for manipulation.
- A laparoscope was inserted via the umbilicus, and dissection mimicked open surgical techniques.
Main Results:
- The two-handed laparoscopic surgery was successfully and safely completed.
- The procedure was performed similarly to open surgery.
- Significant reduction in surgery time was observed.
Conclusions:
- Two-handed laparoscopy is a feasible and safe surgical approach.
- This technique may be beneficial for procedures currently requiring open surgery.
- Further research and clinical trials are warranted to validate these findings.

