Perfusive ion-exchange chromatographic materials with high capacity

Area of Science:

• Chromatography
• Materials Science
• Chemical Engineering

Background:

• Developing advanced chromatographic stationary phases is crucial for efficient separation processes.
• Existing materials often face trade-offs between mass transfer rates and binding capacities.
• Novel materials are needed to overcome these limitations in bioseparations and purification.

Purpose of the Study:

• To characterize novel macro-porous chromatographic stationary phases.
• To evaluate their performance in terms of porosity, HETP, resolution, and binding capacity.
• To compare these new phases against commercially available alternatives.

Main Methods:

• Utilized reactive gelation under shear to synthesize macro-porous particles (100 nm to several microns).
• Characterized particle porosity, hydrodynamic equivalent theoretical plate height (HETP), resolution, and binding capacity.
• Functionalized pore surfaces with charged polymeric brushes to enhance binding.

Main Results:

• Achieved perfusive flow fraction values between 0.02 and 0.01, significantly increasing mass transport.
• Obtained high binding capacities ranging from 25 to 140 mg/mL col. due to functionalized pore surfaces.
• Demonstrated superior resolution performance and dynamic binding capacity compared to Poros 50HS and Fractogel EMD SO3 (M), even at high flow rates.

Conclusions:

• The novel macro-porous stationary phases exhibit enhanced chromatographic performance.
• These materials effectively combine low mass transfer resistance with high binding capacity.
• The developed stationary phases represent a significant advancement over current commercial options for demanding separation tasks.