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Nick translation of lambda phage DNA with a deoxycytidine analog spin labeled in the 5 position
O K Strobel1, E V Bobst, A M Bobst
1Department of Chemistry, University of Cincinnati, Ohio 45221.
Archives of Biochemistry and Biophysics
|September 1, 1989
Abstract:
The synthesis and properties of a novel C(5)-spin-labeled 2'-deoxycytidine 5'-triphosphate which serves as a suitable substrate for the template-directed enzyme Escherichia coli DNA polymerase I are reported. The spin label is readily incorporated into lambda phage DNA by nick translation where it reports the characteristic local base motion for double- and single-stranded DNA as determined by electron spin resonance. The high-frequency deoxycytidine motion is similar to the previously reported thymidine motion in double-stranded lambda phage DNA.