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Hemagglutination with pseudorabies virus. Brief report.

N Tetsu1, Y Inaba, M Yukawa

  • 1Department of Veterinary Epizootiology, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan.

Archives of Virology
|January 1, 1989
PubMed
Summary

Pseudorabies virus exhibits hemagglutination (HA) with mouse erythrocytes, but not other species. Hemagglutination inhibition (HI) antibody titers correlate with neutralizing antibody titers in pigs.

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Area of Science:

  • Veterinary Virology
  • Immunology

Background:

  • Pseudorabies virus (PRV) is a significant pathogen in swine.
  • Understanding PRV interactions with host cells is crucial for diagnostics and vaccine development.

Purpose of the Study:

  • To investigate the hemagglutination (HA) properties of Pseudorabies virus.
  • To establish standardized hemagglutination inhibition (HI) tests for PRV antibody detection.

Main Methods:

  • PRV was cultured in CPK cells.
  • Hemagglutination assays were performed using erythrocytes from various animal species at different temperatures (4°C, 25°C, 37°C).
  • Hemagglutination inhibition (HI) tests were conducted using specific antisera.

Main Results:

  • PRV demonstrated hemagglutination activity exclusively with mouse erythrocytes across all tested temperatures.

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  • Erythrocyte agglutinability in mice showed strain variation, necessitating mouse selection.
  • The HA reaction was effectively inhibited by specific antiserum.
  • Established HA and HI tests showed a significant positive correlation between HI antibody titers and neutralizing antibody titers in individual pig sera.

    • Conclusions:

    • Mouse erythrocytes are suitable for Pseudorabies virus HA testing.
    • Standardized HA and HI assays can be reliably used for PRV antibody detection in pigs.
    • HI antibody titers serve as a reliable indicator of neutralizing antibody levels against PRV.