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Hemagglutination with pseudorabies virus. Brief report
1Department of Veterinary Epizootiology, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan.
Abstract:
Pseudorabies virus grown in CPK cell cultures was tested for hemagglutination (HA) with erythrocytes of a variety of species at 4 degrees C, 25 degrees C and 37 degrees C. HA was observed at all temperatures with mouse erythrocytes but not with cattle, sheep, goat, swine, cat, rabbit, guinea pig, rat, mongolian gerbil, chicken, and goose erythrocytes. Mice showed a strain variation in agglutinability of their erythrocytes, requiring selection of mice to obtain erythrocytes for HA. The HA reaction was inhibited by specific antiserum. Some factors involved in the HA and HA-inhibition (HI) were investigated and standard HA and HI tests were established. HI antibody titers of individual pig sera showed a significant positive correlation with their neutralizing antibody titers.
Insights
Pseudorabies virus exhibits hemagglutination (HA) with mouse erythrocytes, but not other species. Hemagglutination inhibition (HI) antibody titers correlate with neutralizing antibody titers in pigs.
Area of Science:
- Veterinary Virology
- Immunology
Background:
- Pseudorabies virus (PRV) is a significant pathogen in swine.
- Understanding PRV interactions with host cells is crucial for diagnostics and vaccine development.
Purpose of the Study:
- To investigate the hemagglutination (HA) properties of Pseudorabies virus.
- To establish standardized hemagglutination inhibition (HI) tests for PRV antibody detection.
Main Methods:
- PRV was cultured in CPK cells.
- Hemagglutination assays were performed using erythrocytes from various animal species at different temperatures (4°C, 25°C, 37°C).
- Hemagglutination inhibition (HI) tests were conducted using specific antisera.
Main Results:
- PRV demonstrated hemagglutination activity exclusively with mouse erythrocytes across all tested temperatures.
- Erythrocyte agglutinability in mice showed strain variation, necessitating mouse selection.
- The HA reaction was effectively inhibited by specific antiserum.
- Established HA and HI tests showed a significant positive correlation between HI antibody titers and neutralizing antibody titers in individual pig sera.
Conclusions:
- Mouse erythrocytes are suitable for Pseudorabies virus HA testing.
- Standardized HA and HI assays can be reliably used for PRV antibody detection in pigs.
- HI antibody titers serve as a reliable indicator of neutralizing antibody levels against PRV.