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Updated: Apr 19, 2026

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids
Published on: January 7, 2019
Cultivation of microalgal Chlorella for biomass and lipid production using wastewater as nutrient resource
Sheng-Yi Chiu1, Chien-Ya Kao2, Tsai-Yu Chen3
1Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan; Water Technology Division, Material and Chemical Research Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan.
Abstract:
Using wastewater for microalgal cultures is beneficial for minimizing the use of freshwater, reducing the cost of nutrient addition, removing nitrogen and phosphorus from wastewater and producing microalgal biomass as bioresources for biofuel or high-value by-products. There are three main sources of wastewater, municipal (domestic), agricultural and industrial wastewater, which contain a variety of ingredients. Some components in the wastewater, such as nitrogen and phosphorus, are useful ingredients for microalgal cultures. In this review, the effects on the biomass and lipid production of microalgal Chlorella cultures using different kinds of wastewater were summarized. The use of the nutrients resource in wastewater for microalgal cultures was also reviewed. The effect of ammonium in wastewater on microalgal Chlorella growth was intensively discussed. In the end, limitations of wastewater-based of microalgal culture were commented in this review article.
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