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Updated: Apr 19, 2026
Tissue Engineering of the Intestine in a Murine Model
Published on: December 1, 2012
[New technique for intestinal lengthening -- from the idea to the clinical application]
Tamás Cserni1, Garbriella Varga2, Dániel Erces2
1Szegedi Tudományegyetem Sebészeti Műtéttani Intézet 6720 Szeged Pécsi u. 6. Jósa András Megyei Kórház Gyermekosztály Nyíregyháza Royal Manchester Children's University Hospital United Kingdom.
Introduction:
In severe short bowel syndrome, as a result of the natural adaptation, the bowel becomes overdilated, this interferes with the persitalsis and may lead to stasis, bacterial translocation and sepsis. At present two techniques are used to improve peristalsis. The Bianchi procedure is technically challenging, the Serial Transverse Enteroplasty (STEP) is easy however it results in an aphysiological ultrastructure altering the orientation of the muscle fibres. Our aim was to develop an easy technique, which does not alter intestinal muscular ultrastructure dramatically.
Material And Methods:
The idea, Spiral Intestinal Lengthening and Tailoring (SILT), is based on a spiral shape incision of the intestine and retubularisation in a longer but narrower fashion. The feasibility and the effect on the muscular ultrastructure were tested on bowelsimulator and porcine intestine. The intramural microcirculation was checked with intravital microscopy. The outcome was assessed on minipigs (n = 6) than clinical application was commenced.
Results:
SILT was feasible, did not change the orientation of muscle fibres significantly, did not compromised microcirculation, no surgical complication was noted when tailoring did not exceed 75%. The first clinical application was successful.
Conclusion:
SILT is a safe and easy technique and not altering the intestinal musculature significantly.
