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Updated: Apr 19, 2026
Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva
Published on: April 3, 2018
MicroRNA expression in inflamed and noninflamed gingival tissues from Japanese patients
Yorimasa Ogata1, Sari Matsui, Ayako Kato
1Department of Periodontology, Nihon University School of Dentistry at Matsudo.
Abstract:
Periodontitis is a chronic inflammatory disease caused by specific bacteria and viruses. Local, systemic, and environmental factors affect the rate of disease progression. Immune responses to bacterial products, and the subsequent production of inflammatory cytokines, are crucial in the destruction of periodontal tissue. MicroRNAs (miRNAs) are a class of small RNAs that control various cell processes by negatively regulating protein-coding genes. In this study, we compared miRNA expression in inflamed and noninflamed gingival tissues from Japanese dental patients. Total RNAs were isolated from inflamed and noninflamed gingival tissues. miRNA expression profiles were examined by an miRNA microarray, and the data were analyzed by GeneSpring GX, Ingenuity Pathways Analysis, and the TargetScan databases. Observed miRNA expression levels in inflamed gingiva were confirmed by real-time PCR. The three most overexpressed (by >2.72-fold) miRNAs were hsa-miR-150, hsa-miR-223, and hsa-miR-200b, and the three most underexpressed (by <0.39-fold) miRNAs were hsa-miR-379, hsa-miR-199a-5p, and hsa-miR-214. In IPA analysis, hsa-miR-150, hsa-miR-223, and hsa-miR-200b were associated with inflammatory disease, organismal injury, abnormalities, urological disease, and cancer. The present findings suggest that miRNAs are associated with chronic periodontitis lesions in Japanese.
Insights
MicroRNAs (miRNAs) play a role in chronic periodontitis. This study identified specific overexpressed and underexpressed miRNAs in inflamed gingival tissues from Japanese patients, suggesting their association with the disease.
Area of Science:
- Oral biology
- Molecular biology
- Genetics
Background:
- Periodontitis is a chronic inflammatory disease impacting periodontal tissue.
- Immune responses and inflammatory cytokines are key in disease progression.
- MicroRNAs (miRNAs) regulate cellular processes by controlling gene expression.
Purpose of the Study:
- To compare miRNA expression profiles in inflamed versus noninflamed gingival tissues.
- To identify specific miRNAs associated with chronic periodontitis in Japanese patients.
Main Methods:
- RNA isolation from gingival tissues.
- miRNA expression profiling using microarray analysis.
- Data analysis with GeneSpring GX, IPA, and TargetScan; confirmation by real-time PCR.
Main Results:
- Identified significant differences in miRNA expression between inflamed and noninflamed tissues.
- Top overexpressed miRNAs: hsa-miR-150, hsa-miR-223, hsa-miR-200b.
- Top underexpressed miRNAs: hsa-miR-379, hsa-miR-199a-5p, hsa-miR-214.
Conclusions:
- Certain miRNAs are differentially expressed in chronic periodontitis lesions.
- Overexpressed miRNAs (hsa-miR-150, hsa-miR-223, hsa-miR-200b) are linked to inflammatory and other diseases.
- Findings suggest miRNAs are involved in the pathogenesis of periodontitis in Japanese individuals.
