Hydrolysate from eggshell membrane ameliorates intestinal inflammation in mice

Area of Science:

• Biochemistry
• Immunology
• Gastroenterology

Background:

• Inflammatory bowel diseases (IBD), including ulcerative colitis and Crohn's disease, are chronic inflammatory conditions.
• Bioactive peptides and hydro lysates from natural sources are explored for anti-inflammatory properties.
• Eggshell membrane (ESM) is a natural source of bioactive compounds.

Purpose of the Study:

• To investigate the anti-inflammatory activity of eggshell membrane hydro lysate (AL-PS).
• To evaluate AL-PS efficacy in vitro and in vivo models of inflammation.

Main Methods:

• In vitro assays to measure pro-inflammatory cytokine IL-8 secretion.
• In vivo studies using dextran sodium sulphate (DSS)-induced colitis model in animals.
• Assessment of clinical signs, weight loss, and cytokine levels (IL-6, IL-10).

Main Results:

• AL-PS inhibited IL-8 secretion in vitro.
• AL-PS treatment reduced DSS-induced weight loss and colitis symptoms in vivo.
• AL-PS down-regulated IL-6 and IL-10 secretion, indicating reduced intestinal inflammation.

Conclusions:

• Eggshell membrane hydro lysate (AL-PS) demonstrates significant anti-inflammatory effects.
• AL-PS shows potential as a novel preventative therapeutic agent for inflammatory bowel diseases.