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Updated: Apr 19, 2026

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method
Published on: August 6, 2016
Accurate quantification of episomal HIV-1 two-long terminal repeat circles by use of optimized DNA isolation and
Eva Malatinkova1, Maja Kiselinova1, Pawel Bonczkowski1
1HIV Translational Research Unit, Department of Internal Medicine, Faculty of Medicine and Health Sciences, Ghent University and Ghent University Hospital, Ghent, Belgium.
Abstract:
Episomal HIV-1 two-long terminal repeat (2-LTR) circles are considered markers for ongoing viral replication. Two sample processing procedures were compared to accurately quantify 2-LTR in patients by using droplet digital PCR (ddPCR). Here, we show that plasmid isolation with a spiked non-HIV plasmid for normalization enables more accurate 2-LTR quantification than genomic DNA isolation.
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