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Updated: Apr 19, 2026
Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
Published on: March 29, 2017
One-by-one single-molecule detection of mutated nucleobases by monitoring tunneling current using a DNA tip
Phuc Tan Bui1, Tomoaki Nishino, Hiroshi Shiigi
1Department of Materials Science, Osaka Prefecture University, Sakai, Osaka 599-8570, Japan.
Abstract:
A DNA molecule was utilized as a probe tip to achieve single-molecule genetic diagnoses. Hybridization of the probe and target DNAs resulted in electron tunneling along the emergent double-stranded DNA. Simple stationary monitoring of the tunneling current leads to single-molecule DNA detection and discovery of base mismatches and methylation.
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