Related Experiment Video
Updated: Apr 19, 2026

Analyzing the Photo-oxidation of 2-propanol at Indoor Air Level Concentrations Using Field Asymmetric Ion Mobility Spectrometry
Published on: June 14, 2018
Virtues and limitations of Pittsburgh green for ozone detection
Carlos Castelló Beltrán1, Elliott A Palmer, Benjamin R Buckley
1Department of Chemistry, School of Science, Loughborough University, Leicestershire, LE11 3TU, UK. b.r.buckley@lboro.ac.uk.
Abstract:
A recently proposed 2',7'-dichlorofluorescein (DCF)-derived fluorescent probe for the detection of ozone shows good selectivity against a number of reactive oxygen species and good pH stability for biological and environmental applications. It is found, however, that over oxidation of the fluorescent product (Pittsburgh green) can occur. This could render quantitative measurements inaccurate due to a reduction in fluorescence and overlapping fluorescence signals from over oxidation by-products and it requires careful experimental design. Although difficult to assess by fluorescence measurements, the over oxidation can be conveniently monitored by (1)H NMR spectroscopy.
Related Concept Videos
Gas Chromatography: Types of Detectors-II
Atomic Emission Spectroscopy: Lab
High-Performance Liquid Chromatography: Types of Detectors
Gas Chromatography: Overview of Detectors
A non-destructive detector allows a sample to be analyzed without altering or consuming it, meaning the sample can be collected after detection for further analysis. Examples include thermal conductivity detectors and...
Atomic Absorption Spectroscopy: Lab
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...
Gas Chromatography: Types of Detectors-I
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...

