Tff3 is expressed in neurons and microglial cells
Ting Fu1, Anne Stellmacher, Eva B Znalesniak
1Institute of Molecular Biology and Medicinal Chemistry, Otto-von-Guericke University Magdeburg, Magdeburg, Germany.
Summary
Trefoil factor 3 (TFF3) is expressed in rat neurons and activated microglial cells in the brain. This finding suggests novel roles for TFF3 in neuroinflammation, synaptic plasticity, and psychiatric disorders.
Area of Science:
- Neuroscience
- Immunology
- Molecular Biology
Background:
- Trefoil factor family (TFF) peptide TFF3 is secreted by mucous epithelia.
- TFF3 is also found in the immune system and brain.
Purpose of the Study:
- To identify cerebral cell types expressing TFF3.
- To investigate the role of TFF3 in the central nervous system (CNS).
Main Methods:
- Primary cultures of rat cerebral cortex and hippocampus.
- Reverse transcription-polymerase chain reaction (RT-PCR).
- Immunofluorescence and immunocytochemistry.
Main Results:
- TFF3 transcripts detected in neural and glial-enriched cultures.
- TFF3 co-localized with Map2 (neurons) and Iba-1 (activated microglia).
- Neuronal TFF3 expression confirmed in adult rat cerebellum.
Conclusions:
- Neuronal TFF3 suggests a neuropeptide function in fear, memory, depression, and motor skills.
- First demonstration of TFF3 expression in activated microglial cells.
- TFF3 may play a role in CNS immune reactions, synaptic plasticity, cognition, neuroinflammation, and psychiatric disorders.
More Related Videos
10:43Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry
Published on: June 22, 2017
25.5K
12:07A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation
Published on: September 19, 2010
24.7K