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Related Concept Videos

Asthma: Pathogenesis and Management01:20

Asthma: Pathogenesis and Management

1.8K
Asthma is a chronic pulmonary condition involving inflammation of the airways, hyper-reactivity, and reversible obstruction of the airways. This condition can significantly impact a person's quality of life, making breathing difficult and leading to distressing symptoms.
Asthma is classified as allergic and non-allergic. Allergens such as dust mites, pollen, and pet dander trigger allergic asthma, while factors like cold air, intense emotions, or exercise can induce non-allergic asthma.
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Asthma-II: Pathophysiology and Classification01:26

Asthma-II: Pathophysiology and Classification

5.0K
Asthma is a prevalent chronic respiratory condition marked by inflammation and hyperresponsiveness of the airways. Its pathophysiology involves complex interactions among inflammatory pathways, immune responses, and neural mechanisms.
Additionally, environmental and genetic factors play crucial roles in determining an individual's susceptibility to asthma and the severity of their condition.
Critical processes in asthma pathophysiology include:
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Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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COPD: Management Using Bronchodilators and Corticosteroids01:26

COPD: Management Using Bronchodilators and Corticosteroids

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Chronic obstructive pulmonary isease (COPD) involves a group of progressive lung disorders characterized by persistent airflow limitation and chronic respiratory symptoms. Asthma-COPD Overlap Syndrome (ACOS), encompassing features of both asthma and Chronic obstructive pulmonary disease (COPD), is a group of progressive lung disorders that includes chronic bronchitis, emphysema, and refractory (non-reversible) asthma. ACOS leads to complex clinical presentations that combine the inflammatory...
1.2K
Chronic Obstructive Pulmonary Disease-II: Pathophysiology01:20

Chronic Obstructive Pulmonary Disease-II: Pathophysiology

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Chronic Obstructive Pulmonary Disease (COPD) pathophysiology is intricate and multifaceted, involving a complex interplay of physiological processes. Understanding these mechanisms is crucial for effectively managing and treating COPD. Here is an in-depth look at the critical elements in the pathophysiology of COPD:
Chronic Inflammation
5.3K
COPD: Pathogenesis and Clinical Features01:20

COPD: Pathogenesis and Clinical Features

2.3K
Chronic obstructive pulmonary disease (COPD) is a group of lung conditions that progressively worsen over time, including chronic bronchitis and emphysema. This cluster of diseases collectively leads to a gradual and irreversible decline in lung function over time.
The primary cause for the onset of COPD is cigarette smoking and exposure to air pollution. These hazardous factors initiate a chain reaction within the lungs, resulting in chronic inflammation, damage to the airways, and a...
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Related Experiment Video

Updated: Apr 19, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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Asthma and COPD proteomics: current approaches and future directions.

Rosa Terracciano1, Girolamo Pelaia, Mariaimmacolata Preianò

  • 1Department of Health Sciences, "Magna Graecia" University of Catanzaro, Catanzaro, Italy.

Proteomics. Clinical Applications
|December 16, 2014
PubMed
Summary

Proteomics offers new ways to understand asthma and chronic obstructive pulmonary disease (COPD) by analyzing proteins in samples like sputum and blood. This can help improve diagnosis and treatment for these common respiratory diseases.

Keywords:
AsthmaBALFCOPDInduced sputum

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Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
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Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels
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Area of Science:

  • Pulmonary Medicine
  • Biochemistry
  • Analytical Chemistry

Background:

  • Asthma and COPD are common chronic respiratory diseases with complex, not fully understood, pathobiology.
  • Current differentiation relies on clinical and lung function, risking misclassification and suboptimal management.
  • Proteomics, particularly mass spectrometry (MS), has advanced significantly, enabling deep protein analysis.

Purpose of the Study:

  • To summarize recent proteomics investigations in asthma and COPD.
  • To highlight challenges and benefits of using proteomics for phenotypic stratification.
  • To discuss issues in sampling and processing for proteomic analysis of respiratory specimens.

Main Methods:

  • Review of recent proteomics-based investigations.
  • Focus on mass spectrometry (MS) for protein identification and quantification.
  • Analysis of various biological specimens: induced sputum, exhaled breath condensate, epithelial lining fluid, lavage fluids, plasma, and serum.

Main Results:

  • MS enables sensitive, accurate, and rapid identification and quantification of thousands of proteins.
  • Proteomic analysis can reveal qualitative-quantitative differences between diseased and normal states.
  • Standardization of preanalytical sampling procedures remains a key challenge.

Conclusions:

  • Proteomics holds promise for improving the phenotypic stratification of asthma and COPD.
  • Addressing challenges in standardization is crucial for realizing the full potential of proteomic studies.
  • Further research is needed to integrate proteomic findings into clinical practice for better patient outcomes.