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SILAC for biomarker discovery.

Gunnar Dittmar1, Matthias Selbach

  • 1Max Delbrück Center for Molecular Medicine, Berlin, Germany.

Proteomics. Clinical Applications
|December 16, 2014
PubMed
Summary
This summary is machine-generated.

Stable Isotope Labeling by Amino acids in Cell culture (SILAC) enables quantitative proteomics. This review covers SILAC methods for biomarker discovery in clinical samples.

Keywords:
MSQuantificationQuantitative proteomicsSILAC

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Area of Science:

  • Proteomics
  • Biomarker Discovery
  • Mass Spectrometry

Background:

  • Stable Isotope Labeling by Amino acids in Cell culture (SILAC) is a quantitative proteomics technique.
  • It relies on metabolic incorporation of isotope-coded amino acids for protein quantification.
  • SILAC has been used for nearly a decade in various research settings.

Purpose of the Study:

  • To review different SILAC-derived methods.
  • To highlight their application in biomarker identification and development.
  • To emphasize the utility of SILAC for clinically relevant samples.

Main Methods:

  • Overview of SILAC technique and its variations.
  • Discussion of adaptations for tissue and human specimens.
  • Focus on quantitative proteomic approaches.

Main Results:

  • SILAC allows quantification of global protein populations.
  • Characteristic protein changes can be identified.
  • SILAC-derived methods are applicable to clinical samples.

Conclusions:

  • SILAC is a powerful tool for quantitative proteomics.
  • Variations extend its applicability to clinical settings.
  • It facilitates the identification and development of novel biomarkers.