Related Experiment Video
Updated: Aug 15, 2026

Tractable Mammalian Cell Infections with Protozoan-primed Bacteria
Published on: April 2, 2013
Application of numerical systematics to the phenotypic differentiation of legionellae
1Department of Microbiology and Immunology, University of South Carolina School of Medicine, Columbia 29208.
Abstract:
A total of 163 strains, including 106 strains of Legionella pneumophila, 28 strains of Tatlockia micdadei, and 29 strains of other legionellae (including members of the proposed genus Fluoribacter), were studied. Ten tests which together could distinguish the genera previously proposed were identified. These tests included catalase-peroxidase, gelatinase, hippurate hydrolysis, starch hydrolysis, medium browning, acetoin production, oxidase, medium fluorescence, colony fluorescence, and the bromcresol purple spot test. T. micdadei strains were strongly catalase positive and bromcresol purple spot test positive and produced acetoin but otherwise were usually inert in the other tests. L. pneumophila and Fluoribacter species could usually be distinguished by strength of catalase activity, blue-white colony fluorescence (if present), and differences in frequency of hippurate hydrolysis, starch hydrolysis, yellow-green medium fluorescence, and, to a lesser extent, oxidase activity. With a simple algorithm and computer program, the overall accuracy was 98.8%.
More Related Videos
12:30Use of Galleria mellonella as a Model Organism to Study Legionella pneumophila Infection
Published on: November 22, 2013
09:12Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
Published on: March 10, 2020
Related Concept Videos
Evolutionary Relationships through Genome Comparisons
Microbial Classification System
Methods of Classification and Identification
Modern Molecular Taxonomy
Applications of Molecular Taxonomy
Phylogenetic Species Concept in Microbiology