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Updated: Aug 8, 2026

Generation of Human CD40-activated B cells
Published on: October 17, 2009
Leukotriene B4 enhances activation, proliferation, and differentiation of human B lymphocytes
K A Yamaoka1, H E Claésson, A Rosén
1Department of Medical Cell Genetics, Karolinska Institute, Stockholm, Sweden.
Abstract:
Highly purified human tonsillar B lymphocytes at different stages of activation were incubated with leukotriene B4 (LTB4). As a key marker for activation, we used the CD23 Ag. LTB4 enhanced the CD23 expression on resting B cells in synergy with B cell-stimulating factors from 4% to 50%. Maximal effect of LTB4 was observed at 10(-10) M to 10(-12) M. LTB4 also augmented the S and M phase entries as well as Ig secretion in synergy with IL-2 and IL-4. In contrast, 5S,12S-dihydroxyeicosatetraenoic acid, an isomer of LTB4, and leukotriene C4 lacked these effects. The results indicate that LTB4 amplifies lymphokine-driven activation, replication, and differentiation of human B lymphocytes.
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