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B Cell Activation and Differentiation

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The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...

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Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

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B-cell subpopulations in children: National reference values.

Marie Duchamp¹, Delphine Sterlin¹, Aminata Diabate¹

¹Study Center of Primary Immunodeficiencies, Assistance Publique-Hôpitaux de Paris (APHP), Necker Hospital Paris, France.

Immunity, Inflammation and Disease

|December 16, 2014

Summary

This study establishes national reference values for pediatric B-cell subpopulations, crucial for diagnosing primary antibody deficiencies (PADs) and other B-cell immunodeficiencies in children.

Keywords:

B-cells children primary immunodeficiency reference values

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Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Published on: April 16, 2013

Area of Science:

Immunology
Pediatrics
Hematology

Background:

Peripheral B-lymphocytes mature and differentiate post-antigen exposure.
Primary antibody deficiencies (PADs) are linked to B-cell development abnormalities.
Updated classifications emphasize B-cell immunophenotyping and age-specific reference intervals for diagnosing primary immunodeficiencies (PIDs).

Purpose of the Study:

To establish national reference values for memory B-cell subpopulations in healthy children.
To provide age-specific data on naive, switched, and non-switched memory B-cells.
To aid in the screening and diagnosis of pediatric B-cell immunodeficiencies.

Main Methods:

Analyzed peripheral blood from 242 healthy children across seven age groups.
Utilized CD27 and surface IgD expression for B-cell subpopulation analysis.
Determined absolute counts and percentages of naive and memory B-cells.

Main Results:

Naive B-cell percentage decreased from 6 months to 8 years, then stabilized around 70-80%.
Memory B-cells were present at birth, increasing throughout childhood.
Memory B-cell numbers stabilized between 12 and 18 years of age.

Conclusions:

Established national reference intervals for pediatric B-cell levels.
These reference values are essential for diagnosing B-cell immunodeficiencies in children.
The study facilitates immunological diagnosis by providing crucial pediatric reference data.