Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Simplified Derivation of Stable Early Passage Murine Embryonic and Parthenogenetic Stem Cells.

Stem cells and development·2026

Same author

Sensitive detection of cancer antigens enabled by user-defined peptide libraries.

Nature biotechnology·2026

Same author

Exploring the effects of tinzaparin and cisplatin on lung cancer cells in vitro.

Cancer cell international·2026

Same author

Heterologous immunization modulates B-cell epitope competition between helper peptides and the MPER segment in MPER/liposome vaccines.

NPJ vaccines·2026

Same author

Association of specific HLA alleles in patients with interstitial cystitis suggesting autoimmunity.

Frontiers in molecular medicine·2025

Same author

Cancers modulate p53 truncal neoantigen display to evade T cell detection.

bioRxiv : the preprint server for biology·2025

Search research articles

Related Experiment Video

Updated: Apr 19, 2026

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Published on: November 2, 2013

Human leukocyte antigen typing using a knowledge base coupled with a high-throughput oligonucleotide probe array

Guang Lan Zhang¹, Derin B Keskin², Hsin-Nan Lin³

¹Cancer Vaccine Center, Dana-Farber Cancer Institute, Harvard Medical School , Boston, MA , USA ; Department of Computer Science, Metropolitan College, Boston University , Boston, MA , USA.

Frontiers in Immunology

|December 16, 2014

Summary

This summary is machine-generated.

This study introduces a novel DNA microarray for human leukocyte antigen (HLA) typing, offering a faster and more comprehensive alternative to current methods. This high-throughput approach enhances HLA typing for global health and personalized medicine.

Keywords:

HLA disease association HLA typing population typing

More Related Videos

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation

Published on: September 6, 2017

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Related Experiment Videos

Last Updated: Apr 19, 2026

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Published on: November 2, 2013

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation

Published on: September 6, 2017

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Area of Science:

Immunogenetics
Molecular Diagnostics
Biotechnology

Background:

Human leukocyte antigens (HLA) are critical biomarkers for diseases, drug responses, and vaccine efficacy.
Current clinical HLA typing methods are time-consuming elimination processes requiring serial testing.

Purpose of the Study:

To develop and validate an alternative in situ synthesized DNA-based microarray for high-throughput HLA typing.
To achieve 4-digit resolution for clinically relevant HLA class I alleles.

Main Methods:

Development of a DNA microarray with hundreds of thousands of probes covering 1,610 HLA class I alleles.
Utilized computational tools for assigning HLA types.
Proof-of-concept validation using 21 blood samples, 18 cell lines, and controls.

Main Results:

The DNA microarray method demonstrated accuracy and robustness, amenable to automation.
Typing errors were minimal, primarily in homozygous or closely related alleles, resolvable by DNA sequencing.
Achieved 4-digit resolution for HLA typing.

Conclusions:

The novel DNA microarray offers an effective, inexpensive, and high-throughput alternative for HLA typing.
This technology can facilitate large-scale population studies, advancing global public health and personalized healthcare.