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Related Experiment Videos

Elastonectin and the elastin receptor.

L Robert1, M P Jacob, T Fülop

  • 1Laboratoire de Biochimie du Tissu Conjonctif, UA CNRS, Faculté de Médecine, Créteil, France.

Pathologie-Biologie
|June 1, 1989
PubMed
Summary
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Cells interact with elastin via distinct mechanisms: direct adhesion mediated by elastonectin and soluble peptide binding by elastin receptors. These pathways, involving G-proteins and calcium signaling, differ in normal and transformed cells.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Medicine

Background:

  • Cells interact with elastin through distinct mechanisms, including adhesion to insoluble fibers and binding of soluble peptides.
  • These interactions are crucial for various cellular functions and can differ between normal and transformed cells.

Purpose of the Study:

  • To elucidate the distinct mechanisms of cell-elastin interactions.
  • To identify key proteins involved in cell adhesion to elastin.
  • To characterize elastin receptors and their downstream signaling pathways.

Main Methods:

  • Co-isolation of proteins involved in cell adhesion to elastin fibers.
  • Scatchard plot analysis to determine elastin receptor affinity.
  • Investigation of cellular responses triggered by elastin peptide binding, including calcium influx and enzyme release.

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Main Results:

  • Identified a novel cell-membrane complex with a 120 kD protein, termed elastonectin, mediating adhesion to insoluble elastin.
  • Demonstrated distinct mechanisms for elastin fiber adhesion and soluble elastin peptide binding.
  • Characterized high-affinity elastin receptors on mesenchymal cells and white blood cells (nanomolar Kd).
  • Elastin peptide binding triggers chemotaxis, calcium influx, and enzyme/radical release, mediated by G-protein-phospholipase-IP3 signaling.
  • Elastonectin biosynthesis and membrane localization appear to be triggered by elastin receptor signaling.

Conclusions:

  • Cellular interactions with elastin involve at least two distinct mechanisms: elastonectin-mediated adhesion and elastin receptor-mediated peptide binding.
  • Elastin receptors activate intracellular signaling pathways, including calcium mobilization, and influence elastonectin expression.
  • The interplay between elastin receptor signaling and adhesion mechanisms is altered in transformed cells, suggesting implications for cancer metastasis.