[Filling the 3D scaffolds by the stromal bone marrow cells depends on the methods of cell seeding and the scaffold...

The distribution of bone marrow stromal cells (BMSC) is studied in a 3D poly(L,L-lactide) scaffolds. It is shown that the population of cells seeding into the scaffold with a peristaltic pump (dynamic method) allows the penetration of cells inside of the scaffold compared with the application of the cell suspension on the scaffold surface (static method). In contrast to cells seeding to scaffold by dynamic method the cells seeding by static method migrate from scaffolds in the first few days almost completely. It is found that BMSCs cultured in 3D polylactide scaffold modified by fibrin form colonies, while BMSCs cultured in 3D polylactide-scaffold modified by collagen type I distribute inside scaffold such single cells.