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Evidence that v-src and v-fps gene products use a protein kinase C-mediated pathway to induce expression of a

R Spangler1, C Joseph, S A Qureshi

  • 1Institute for Biomolecular Structure and Function, Hunter College, City University of New York, NY 10021.

Proceedings of the National Academy of Sciences of the United States of America
|September 1, 1989
PubMed

Insights

The study reveals that protein kinase C (PKC) is crucial for the v-Src and v-Fps oncogenes to induce the transformation-related gene 9E3. This suggests a PKC-mediated signaling pathway is involved in cancer-related gene expression.

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Oncogenesis

Background:

  • The v-src and v-fps oncogenes are known to induce cellular transformation.
  • The transformation-related gene 9E3 is implicated in cellular processes.
  • Protein kinase C (PKC) is a family of enzymes involved in various cellular signaling pathways.

Purpose of the Study:

  • To investigate the role of protein kinase C (PKC) in the induction of the 9E3 gene by v-Src and v-Fps oncogenes.
  • To elucidate the signal-transduction pathway utilized by v-Src and v-Fps for 9E3 gene expression.

Main Methods:

  • Depletion of protein kinase C (PKC) in chicken embryo fibroblasts.
  • Treatment with PKC agonists and protein kinase inhibitors.
  • Analysis of 9E3 gene expression.
  • Assay for phosphorylation of a 67-kDa PKC substrate.

Main Results:

  • Depletion of PKC blocked v-Src- and v-Fps-induced 9E3 gene expression.
  • PKC agonists induced 9E3 gene expression.
  • Protein kinase inhibitors mimicked the dose-response of PKC agonists in blocking v-Src- and v-Fps-induced 9E3 expression.
  • Both v-Src and v-Fps rapidly induced phosphorylation of a 67-kDa PKC substrate.

Conclusions:

  • v-Src and v-Fps oncogenes utilize a protein kinase C (PKC)-mediated signal-transduction pathway to induce the transformation-related 9E3 gene.
  • PKC activation is a key event in the signaling cascade initiated by v-Src and v-Fps leading to 9E3 gene expression.

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