Related Experiment Video
Updated: Apr 19, 2026

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
Published on: March 25, 2020
[Substrate specificity and properties of methyl-directed site-specific dna endonucleases]
Abstract:
Methyl-directed site-specific DNA endonucleases (MD endonucleases) form a small group of enzymes which specifically cleave only methylated DNA. There are N6-methyladenine- and 5-methylcytosine-directed enzymes in this group. In spite of limited information on the MD endonucleases they are considered to be a very interesting subject for both fundamental investigations and practical use in biotechnology and epigenomics. In this review for the first time the data on properties of MD endonucleases are summarized in particular on substate specificity of these enzymes. The role of MD endonucleases in bacterial cells and the potential of their practical use are also discussed.
More Related Videos
05:33Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication
Published on: July 5, 2024
06:07Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
Published on: August 5, 2022
Related Concept Videos
Restriction Enzymes
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
Single-Strand DNA Binding Proteins
Mismatch Repair
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
Maxam-Gilbert Sequencing
Challenges of the Maxam-Gilbert Method
The...