Related Experiment Video
Updated: Apr 19, 2026

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions
Published on: May 27, 2021
Phosphatidylcholine bearing 6,6-dideuterated oleic acid: a useful solid-state (2)H NMR probe for investigating
Jin Cui1, Sébastien Lethu1, Tomokazu Yasuda2
1Department of Chemistry, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan; JST, ERATO, Lipid Active Structure Project, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan; Project Research Centre for Fundamental Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan.
Abstract:
Lipid organization has been at the center of research on lipid rafts. Dioleoylphosphatidylcholine (DOPC) is a typical unsaturated lipid. Very few studies have reported its thermodynamics in raft-like membranes. Herein, we have developed a highly efficient synthetic method for [C6-(2)H2] oleic acid, and newly synthesized [C6-(2)H2] DOPC. In raft-like oriented bilayers, [C6-(2)H2] DOPC shows clear phase separation and characteristic phase behavior at various temperature. It has been successfully utilized for the comparison of membrane properties between sphingomyelin (SM) and dihydrosphingomyelin (DHSM) membranes.
More Related Videos
08:31Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6
Published on: July 29, 2021
10:58SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy
Published on: August 24, 2016
Related Concept Videos
Synthesis of Phosphatidylcholine in the ER Membrane
The major components of all eukaryotic cell...
Asymmetric Lipid Bilayer
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
¹H NMR of Labile Protons: Deuterium (²H) Substitution