Related Experiment Video
Updated: Apr 19, 2026
12:34
Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry
Published on: May 9, 2019
8.2K
Cyclin D1 and Ki-67 expression in normal, hyperplastic and neoplastic endometrium
1Department of Pathology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
Journal of Postgraduate Medicine
|December 17, 2014
Summary
Cyclin D1 and Ki-67 expression increases in endometrial carcinoma compared to normal tissue. These cell cycle proteins correlate positively and may serve as biomarkers for endometrial cancer development and proliferation.
Area of Science:
- Oncology
- Cell Biology
- Gynecologic Pathology
Background:
- Cell cycle regulation is crucial for cancer cell proliferation and differentiation.
- Recent advancements have significantly improved our understanding of cell cycle regulatory proteins.
Purpose of the Study:
- To quantitatively examine cell cycle proteins (Cyclin D1, Ki-67) in normal endometrium, hyperplasias, and endometrial carcinoma.
- To assess the correlation between Cyclin D1 and Ki-67 as proliferation markers.
Main Methods:
- Retrospective case control study.
- Evaluated Cyclin D1 and Ki-67 expression in 61 endometrial samples (hyperplasia and carcinoma).
Main Results:
- Cyclin D1 and Ki-67 expression were elevated in endometrial carcinoma versus normal endometrium and simple hyperplasia.
- No significant difference in expression between atypical hyperplasia and carcinoma.
- Cyclin D1 expression positively correlated with Ki-67 expression.
Conclusions:
- Cyclin D1 and Ki-67 show potential as biomarkers for endometrial carcinogenesis.
- Combined assessment of Cyclin D1 and Ki-67 may indicate tumor cell proliferation.
More Related Videos
Related Concept Videos
02:39Positive Regulator Molecules
7.1K
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
7.1K
01:45Positive Regulator Molecules
137.4K
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
137.4K
02:34Inhibition of Cdk Activity
6.3K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
6.3K
01:26Cellular Adaptation III: Hyperplasia
8
Hyperplasia is an increase in the number of cells in a tissue or organ due to enhanced cell division. It is an adaptive, controlled response to stimuli such as injury, hormones, or stress, involving mitosis to produce genetically identical cells and support tissue repair and regeneration.Tissue CapacityCertain tissues, including the epidermis, intestinal epithelium, bone marrow, and fibroblasts, have a high potential for hyperplasia. Others, such as bone, cartilage, and smooth muscle, show...
8
01:24Cellular Adaptation IV: Dysplasia and Metaplasia
4
DysplasiaDysplasia refers to abnormal changes in the size, shape, and organization of mature cells, characterized by pleomorphism, nuclear abnormalities, and increased mitotic activity. It commonly affects epithelial tissues, including the cervix, gastrointestinal tract, respiratory mucosa, and endometrium. Although it may occur alongside hyperplasia, dysplasia is not a true adaptive response but a preneoplastic change with potential to progress to cancer.When confined above the basement...
4
02:15M-Cdk Drives Transition Into Mitosis
6.8K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
6.8K