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Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology
Published on: March 22, 2012
[Application of detecting mannose-binding lectins in pulmonary aspergillosis in rats]
1Department of Respiratory Medicine, Shandong Chest Hospital, Jinan 250013, China.
Objective:
To explore the effects of mannose-binding lectins (MBL) in pulmonary aspergillosis.
Methods:
A total of 96 adult Wistar rats were randomly divided into 3 groups of aspergillus fumigatus (A.fumigatus) pulmonary, colonization and normal (n = 32 each). An intramuscular injection (0.6 mg/kg × 3 d) of dexamethasone sodium phosphate and a nostril drop (1 × 10⁷ cfu/ml) were administered for modeling. The animals were sacrificed at Days 3, 7, 14 and 28 post-inoculation and blood samples were obtained by cardiac puncture for MBL detection. Lung tissues were prepared for routine pathology examinations. The lung tissues infected with A.fumigatus showed remarkable inflammatory reactions. The serum value of MBL was detected by enzyme-linked immunosorbent assay (ELISA). And the results were compared and analyzed.
Results:
All infected lung tissues showed remarkable inflammatory reactions. The serum MBL levels of A.fumigatus pulmonary and colonization groups were (8.57 ± 0.88) and (7.87 ± 0.45) ng/ml and both significantly increased compared with non-pulmonary aspergillosis group (P < 0.05). The area under the curve was 0.744 (P = 0.002). The diagnosis of MBL had a sensitivity of 75.0%, a specificity of 86.7%, a positive predictive value of 87.1% and a negative predictive value of 74.3% for pulmonary aspergillosis. The inflammatory cells infiltration were detected in pathology in infection and colonization groups at Day 3 and Day 7.
Conclusion:
The detection of serum MBL may be used for the diagnosis of pulmonary aspergillosis.
Insights
Mannose-binding lectin (MBL) levels increase in rats with pulmonary aspergillosis. Serum MBL detection shows potential for diagnosing this fungal lung infection.
Area of Science:
- Immunology
- Mycology
- Pulmonology
Background:
- Pulmonary aspergillosis is a serious fungal infection.
- Mannose-binding lectin (MBL) plays a role in innate immunity.
- Understanding MBL's role can aid in diagnosis and treatment.
Purpose of the Study:
- To investigate the association between serum mannose-binding lectin (MBL) levels and pulmonary aspergillosis.
- To evaluate the diagnostic potential of MBL in pulmonary aspergillosis.
Main Methods:
- Wistar rats were divided into three groups: Aspergillus fumigatus pulmonary infection, colonization, and normal controls.
- Pulmonary aspergillosis was induced using dexamethasone and A. fumigatus inoculation.
- Serum MBL levels were measured using ELISA at various time points post-inoculation.
Main Results:
- Significant increases in serum MBL levels were observed in rats with pulmonary aspergillosis and colonization compared to controls.
- MBL detection demonstrated a sensitivity of 75.0% and specificity of 86.7% for pulmonary aspergillosis.
- Histopathological examination revealed inflammatory cell infiltration in infected lung tissues.
Conclusions:
- Serum MBL levels are elevated in pulmonary aspergillosis.
- MBL detection is a promising biomarker for diagnosing pulmonary aspergillosis.
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