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The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
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Biochemical characterization of the POM-1 metallo-β-lactamase from Pseudomonas otitidis
Luisa Borgianni1, Filomena De Luca1, Maria Cristina Thaller2
1Dipartimento di Biotecnologie Mediche, Università di Siena, Siena, Italy.
Antimicrobial Agents and Chemotherapy
|December 17, 2014
Abstract:
The POM-1 metallo-β-lactamase is a subclass B3 resident enzyme produced by Pseudomonas otitidis, a pathogen causing otic infections. The enzyme was overproduced in Escherichia coli BL21(DE3), purified by chromatography, and subjected to structural and functional analysis. The purified POM-1 is a tetrameric enzyme of broad substrate specificity with higher catalytic activities with penicillins and carbapenems than with cephalosporins.

