Related Experiment Video
Updated: Apr 19, 2026

High-throughput Saccharification Assay for Lignocellulosic Materials
Published on: July 3, 2011
Simultaneous pretreatment and saccharification: green technology for enhanced sugar yields from biomass using a
Saurabh Sudha Dhiman1, Jung-Rim Haw2, Dayanand Kalyani3
1Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea; Institute of SK-KU Biomaterials, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea.
Abstract:
Two different biomasses were subjected to simultaneous pretreatment and saccharification (SPS) using a cocktail of hydrolytic and oxidizing enzymes. Application of a novel laccase as a detoxifying agent caused the removal of 49.8% and 32.6% of phenolic contents from the soaked rice straw and willow, respectively. Hydrolysis of soaked substrates using a newly developed fungal consortium resulted in saccharification yield of up to 74.2% and 63.6% for rice straw and willow, respectively. A high saccharification yield was obtained with soaked rice straw and willow without using any hazardous chemicals. The efficiency of each step related to SPS was confirmed by atomic force microscopy. The suitability of the developed SPS process was further confirmed by converting the hydrolysate from the process into bioethanol with 72.4% sugar conversion efficiency. To the best of our knowledge, this is the first report on the development of a less tedious, single-pot, and eco-friendly SPS methodology.
More Related Videos
Related Concept Videos
Production of Organic Acids
Bioreactor Controls-III
Microbes in Food Production
Biofuels
Microbial Fermentation
Microbes in Beverage Production

