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A T-cell line with an unusual phenotype.
W C Chan1, S Link, A Srinivasan
1Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA.
Cancer
|November 1, 1989
Summary
A novel T-cell line, HCD8, was identified with an unusual phenotype, lacking cytotoxic activity but expressing specific T-cell antigens. This discovery offers insights into T-cell lymphomas and antigen expression.
Area of Science:
- Immunology
- Cell Biology
- Oncology
Background:
- A unique T-cell line, HCD8, was established from healthy donor peripheral blood lymphocytes.
- The HCD8 cell line exhibited an atypical immunophenotype, distinct from typical T-cells.
Purpose of the Study:
- To characterize the phenotype and function of the novel HCD8 T-cell line.
- To investigate the potential relationship between HCD8 cell markers and T-cell lymphomas.
Main Methods:
- Flow cytometry was used to determine T-cell surface marker expression.
- Reverse transcriptase assays and retroviral DNA analysis excluded viral transformation.
- T-cell receptor (TCAR) gene rearrangement studies were performed.
- Cellular differentiation was induced using 12-O-tetradecanoyl phorbol 13-acetate.
Main Results:
- HCD8 cells showed a CD1+, CD2-, CD3+(cytoplasmic), CD4-, CD5+, CD7+, CD8-, IL-2R-, TCAR alpha beta- phenotype.
- No evidence of HTLV or HIV infection was found.
- The cells lacked natural killer (NK) and antibody-dependent cell-mediated cytotoxic (ADCC) functions.
- TCAR gene studies revealed T gamma rearrangement, germline T beta, and deleted T delta genes.
- HCD8 cells strongly expressed Leu M1 and Ki-1 antigens, typically found on lymphoma cells.
Conclusions:
- The HCD8 cell line represents a distinct T-cell population with an unusual phenotype.
- HCD8 cells may serve as a model for studying Leu M1 and Ki-1 antigen expression.
- This cell line could provide insights into the cellular origins of certain T-cell lymphomas.