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Updated: Apr 19, 2026

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

Engineering myocardial tissue patches with hierarchical structure-function.

Erin G Roberts¹, Elaine L Lee, Daniel Backman

¹Division of Materials Science and Engineering, Boston University, 15 St. Mary's St., Boston, MA, 02215, USA.

Annals of Biomedical Engineering

|December 18, 2014

Summary

This summary is machine-generated.

This study reviews methods for creating scaffold-less myocardial patches by layering cell sheets. Integrating computational and experimental approaches optimizes engineered tissue function through iterative design and testing.

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Last Updated: Apr 19, 2026

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

Generation of Aligned Functional Myocardial Tissue Through Microcontact Printing

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Published on: March 19, 2013

Transplantation of a 3D Bioprinted Patch in a Murine Model of Myocardial Infarction

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Published on: September 26, 2020

Area of Science:

Biomedical Engineering
Regenerative Medicine
Tissue Engineering

Background:

Complex hierarchical organization is crucial for tissue and organ development.
A key challenge in tissue engineering is creating structurally organized and functional cell arrays.
Understanding cellular responses to physiochemical cues enables the design of biomimetic tissue structures.

Purpose of the Study:

To review the fabrication and optimization of scaffold-less myocardial patches using layered cell sheets.
To explore the integration of experimental and computational methods for enhancing engineered tissue properties.
To discuss the impact of tissue anisotropy and mechanical stimuli on cell phenotype and tissue function.

Main Methods:

Review of cell sheet layering techniques for myocardial patch fabrication.
Discussion of methods for mechanical conditioning and functional testing of engineered tissues.
Integration of biomechanical property measurements and computational modeling for optimization.

Main Results:

Tissue anisotropy and dynamic mechanical stimuli influence cell phenotype, protein expression, and secretion.
These cellular changes lead to modifications in tissue composition and structure, affecting overall function.
A combinatorial, iterative approach of design, fabrication, testing, and modeling is effective for optimizing engineered tissue function.

Conclusions:

Scaffold-less myocardial patches can be engineered using layered cell sheets.
Integrating computational and experimental approaches is vital for optimizing mechanical properties and function.
Iterative optimization through design, fabrication, testing, and modeling enhances engineered tissue performance.