Related Experiment Video
Updated: Apr 19, 2026
11:23
Phloem Sap Sampling from Brassica napus for 3D-PAGE of Protein and Ribonucleoprotein Complexes
Published on: January 9, 2018
14.7K
Identification of phloem-mobile mRNA
1Graduate School of Science, Nagoya University, B-105, Bldg B, Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan, notaguchi.michitaka@b.mbox.nagoya-u.ac.jp.
Journal of Plant Research
|December 18, 2014
Summary
Plants use plasmodesmata for cell communication. This review explores how messenger RNAs (mRNAs) in the phloem might facilitate long-distance plant signaling, though their exact role remains unclear.
Area of Science:
- Plant biology
- Cellular communication
- Molecular biology
Background:
- Multicellular organisms rely on intercellular signaling for development and adaptation.
- Plants utilize symplasmic (plasmodesmata) and apoplasmic pathways for communication.
- Phloem transports photosynthates, small molecules, proteins, small RNAs, and potentially mRNAs over long distances.
Purpose of the Study:
- To review current methods for identifying mRNA populations in the plant phloem translocation system.
- To discuss the potential roles of short- and long-distance mRNA transport in plant physiology.
Main Methods:
- Summary of existing techniques for phloem sap collection and mRNA profiling.
- Analysis of literature on phloem-mobile molecules and their functions.
Main Results:
- Phloem transport of proteins and small RNAs is established and linked to key processes like flowering and nutrient allocation.
- Mobility of mRNAs within the phloem has been demonstrated.
- The specific biological functions of phloem-transported mRNAs are not yet fully understood.
Conclusions:
- Further investigation into phloem mRNA profiles is crucial for understanding their role in plant signaling.
- mRNA transport may represent an underappreciated mechanism for systemic regulation in plants.
Related Concept Videos
02:24Ribosome Profiling
4.4K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.4K
02:22Regulated mRNA Transport
7.3K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
7.3K
02:22Regulated mRNA Transport
3.6K
3.6K
02:02Phloem and Sugar Transport
41.3K
Like many living organisms, plants have tissues that specialize in specific plant functions. For example, shoots are well adapted to rapid growth, while roots are structured to acquire resources efficiently. However, sugar production is primarily restricted to the photosynthetic cells that reside in the leaves of angiosperm plants. Sugar and other resources are transported from photosynthetic tissues to other specialized tissues by a process called translocation.
41.3K
02:28Leaky Scanning
5.9K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.9K