Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Epilepsy and Seizures: Overview01:24

Epilepsy and Seizures: Overview

1.8K
Epilepsy is a chronic neurological disease marked by recurrent, unpredictable seizures. These seizures are caused by abnormal electrical discharges in the brain, leading to behavior, sensation, or consciousness alterations. They can also cause transient impairment of awareness, interfering with daily activities.
Various factors can trigger epilepsy, including genetic factors, brain damage, metabolic causes, and unknown etiology. Diagnosis of epilepsy involves electroencephalography (EEG), which...
1.8K
iPS Cell Differentiation01:22

iPS Cell Differentiation

3.3K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
3.3K
EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

3.5K
Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
3.5K
Embryonic Stem Cells00:58

Embryonic Stem Cells

33.6K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
33.6K
Embryonic Stem Cells00:57

Embryonic Stem Cells

6.0K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
6.0K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

28.7K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An epilepsy-associated KV3.1 potassium channel variant acts via dominant-positive effect.

The Journal of general physiology·2026
Same author

Compensatory rearrangement of parvalbumin interneuron voltage-gated sodium channel subunits in a mouse model of Dravet syndrome.

Epilepsia·2026
Same author

In vivo adenine base editing ameliorates Dravet syndrome phenotypes in a mouse model.

Science translational medicine·2026
Same author

Genetic testing for familial epilepsies: Diagnostic yield and genetic findings.

Epilepsia·2026
Same author

Impaired excitability of fast-spiking neurons in a novel mouse model of <i>KCNC1</i> epileptic encephalopathy.

eLife·2026
Same author

Use-dependent regulation of the axonal action potential in parvalbumin-expressing interneurons.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Apr 19, 2026

Electrophoretic Delivery of &#947;-aminobutyric Acid GABA into Epileptic Focus Prevents Seizures in Mice
07:01

Electrophoretic Delivery of γ-aminobutyric Acid GABA into Epileptic Focus Prevents Seizures in Mice

Published on: May 16, 2019

9.7K

Seizing the opportunity: stem cells take on epilepsy.

Ethan M Goldberg1, Douglas A Coulter2

  • 1Division of Neurology, The Children's Hospital of Philadelphia, The University of Pennsylvania, Philadelphia, PA 19083, USA; Department of Neurology, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, PA 19083, USA.

Cell Stem Cell
|December 18, 2014
PubMed
Summary

Human embryonic stem cells can generate neurons to treat neurological conditions like epilepsy. Researchers used these stem cells to develop GABAergic interneuron progenitors, successfully treating temporal lobe epilepsy in mice.

More Related Videos

Preparation and Implantation of Electrodes for Electrically Kindling VGAT-Cre Mice to Generate a Model for Temporal Lobe Epilepsy
09:29

Preparation and Implantation of Electrodes for Electrically Kindling VGAT-Cre Mice to Generate a Model for Temporal Lobe Epilepsy

Published on: August 17, 2021

3.0K
Transplantation of Human Stem Cell-Derived GABAergic Neurons into the Early Postnatal Mouse Hippocampus to Mitigate Neurodevelopmental Disorders
05:00

Transplantation of Human Stem Cell-Derived GABAergic Neurons into the Early Postnatal Mouse Hippocampus to Mitigate Neurodevelopmental Disorders

Published on: November 11, 2022

3.1K

Related Experiment Videos

Last Updated: Apr 19, 2026

Electrophoretic Delivery of &#947;-aminobutyric Acid GABA into Epileptic Focus Prevents Seizures in Mice
07:01

Electrophoretic Delivery of γ-aminobutyric Acid GABA into Epileptic Focus Prevents Seizures in Mice

Published on: May 16, 2019

9.7K
Preparation and Implantation of Electrodes for Electrically Kindling VGAT-Cre Mice to Generate a Model for Temporal Lobe Epilepsy
09:29

Preparation and Implantation of Electrodes for Electrically Kindling VGAT-Cre Mice to Generate a Model for Temporal Lobe Epilepsy

Published on: August 17, 2021

3.0K
Transplantation of Human Stem Cell-Derived GABAergic Neurons into the Early Postnatal Mouse Hippocampus to Mitigate Neurodevelopmental Disorders
05:00

Transplantation of Human Stem Cell-Derived GABAergic Neurons into the Early Postnatal Mouse Hippocampus to Mitigate Neurodevelopmental Disorders

Published on: November 11, 2022

3.1K

Area of Science:

  • Neuroscience
  • Stem Cell Biology
  • Epilepsy Research

Background:

  • Stem cells offer potential for neurological condition treatments.
  • Epilepsy, particularly temporal lobe epilepsy, presents significant therapeutic challenges.
  • Replacing lost or dysfunctional neurons is a key goal in regenerative neurology.

Purpose of the Study:

  • To investigate the therapeutic potential of human embryonic stem cell-derived neurons for epilepsy.
  • To assess the efficacy of cortical GABAergic interneuron progenitors in a mouse model of temporal lobe epilepsy.

Main Methods:

  • Derivation of cortical GABAergic interneuron progenitors from human embryonic stem cells.
  • Transplantation of these progenitors into a mouse model of chronic temporal lobe epilepsy.
  • Evaluation of seizure activity and neuronal integration post-transplantation.

Main Results:

  • Successful engraftment and differentiation of transplanted progenitors into functional interneurons.
  • Significant reduction in seizure frequency and severity in treated mice.
  • Demonstration of the therapeutic potential of stem cell-derived neurons in an epilepsy model.

Conclusions:

  • Human embryonic stem cell-derived GABAergic interneurons are a viable option for treating temporal lobe epilepsy.
  • This approach holds promise for regenerative therapies in neurological disorders.
  • Further research is warranted to translate these findings to clinical applications.