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CD200 in growing rat lungs: developmental expression and control by dexamethasone
Mang-Hung Tsai1, Chin-Chen Chu, Tsui-Shan Wei
1Department of Anatomy, China Medical University, Taichung, 404, Taiwan.
Cell and Tissue Research
|December 19, 2014
Summary
Lung CD200 protein, a cell adhesion molecule, shows dynamic expression changes during development in rats. Its localization shifts from diffuse to specific domains on alveolar endothelial cells as lungs mature.
Area of Science:
- Immunology
- Developmental Biology
- Cell Biology
Background:
- CD200 is an immunosuppressive cell adhesion molecule of the immunoglobulin superfamily.
- Previous studies identified CD200 on adult rat alveolar endothelial cells at the blood-air barrier.
Purpose of the Study:
- To investigate the developmental regulation and localization of CD200 in rat lungs.
- To explore the influence of dexamethasone on lung CD200 expression.
Main Methods:
- Double-immunofluorescence staining in fetal and neonatal rat lungs.
- Immunoelectron microscopy to determine subcellular localization.
- Analysis of CD200 protein levels in neonatal rats treated with dexamethasone.
Main Results:
- Lung CD200 protein expression increases with age, peaking in the early postnatal period, then declining and rising again.
- CD200 localization shifts from the entire luminal membrane and cytoplasm in fetal/neonatal lungs to a specific luminal domain in mature lungs.
- Dexamethasone treatment significantly increases lung CD200 levels in neonatal rats.
Conclusions:
- Lung CD200 expression is developmentally regulated in rats.
- Pulmonary microvascular maturation involves the redistribution of CD200 to specific endothelial domains.
- Hormonal factors, such as dexamethasone, may influence CD200 expression during lung development.
