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Updated: Apr 19, 2026
Technical Refinement of a Bilateral Renal Ischemia-Reperfusion Mouse Model for Acute Kidney Injury Research
Published on: November 3, 2023
Protective effect of baicalein on renal ischemia/reperfusion injury in the rat
Kefei Wu1, Hongyan Li, Jianwei Tian
1Department of Nephrology, The First Affiliated Hospital of Shantou University Medical College , Shantou , China .
Objective:
To investigate the protective effect and the mechanism of baicalein (Bai) in rats with renal ischemia-reperfusion injury (RIRI).
Methods:
Twenty-four male Sprague-Dawley rats were divided into three groups: sham, IR, and IR+Bai. Bai was administered by tail vein injection (30 mg/kg) 30 min before reperfusion in the IR+Bai group. The IR group and sham group received saline vehicle via the intravenous route.
Results:
Rats that underwent RIRI exhibited renal functional impairment, histological changes, significantly increased advanced oxidation protein product (AOPP) and malondialdehyde (MDA) levels (p<0.01), and ICAM-1 and MCP-1 protein and mRNA expression were significantly upregulated (p<0.01). Administration of Bai reduced AOPP and MDA levels, significantly inhibited expression of inflammatory factors (p<0.05), and markedly improved renal function.
Conclusion:
Bai promotes the recovery of renal function in established acute RIRI, and alleviates kidney injury in a rat model.
