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High-Throughput Screening of Microbial Isolates with Impact on Caenorhabditis elegans Health

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Microbial shifts in the aging mouse gut.

Morgan Gi Langille¹, Conor J Meehan², Jeremy E Koenig³

¹Department of Pharmacology, Dalhousie University, Halifax, Nova Scotia Canada ; Faculty of Computer Science, Dalhousie University, Halifax, Nova Scotia Canada.

|December 19, 2014

Summary

Aging alters the gut microbiome in mice, with frailty closely mirroring age. Key bacterial functions shift, impacting nutrition and drug metabolism in older animals.

Keywords:

Aging Alistipes B12 Frailty index Mice Microbiome

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Area of Science:

Microbiome research
Aging biology
Gerontology

Background:

Changes in the aging microbiome are not fully understood, especially concerning frailty.
Previous human studies are limited by confounding factors like diet and residence.

Purpose of the Study:

To investigate the relationship between age, frailty, and the gut microbiome.
To utilize a controlled mouse model (female C57BL/6 J mice) for this investigation.

Main Methods:

Developed a frailty index based on 31 clinical signs in mice.
Analyzed the taxonomic and functional composition of the gut microbiome.
Correlated microbiome changes with host age and frailty index.

Main Results:

Frailty index strongly correlated with host age.
Significant age-related shifts in gut microbiome taxonomy (e.g., Rikenellaceae family overrepresentation).
Functional changes included decreased B12/B7 biosynthesis, altered DNA repair genes, increased creatine degradation, and modified carbohydrate utilization.

Conclusions:

Identified specific taxonomic and functional microbiome patterns associated with age and frailty in mice.
Age-dependent functional shifts impact host nutrition and drug pharmacology.
Further research is needed to disentangle age and frailty effects.