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Histopathological biomarkers in juvenile silver catfish (Rhamdia quelen) exposed to a sublethal lead concentration
Lautaro Muñoz1, Paula Weber2, Valderi Dressler3
1Cátedra de Histología y Embriología, Facultad de Ciencias Veterinarias, Universidad Nacional de Rosario, Bv. Ovidio Lagos y Ruta 33, 2170 Casilda, Argentina; Centro de Investigaciones en Piscicultura Experimental (CIPEX), Facultad de Ciencias Veterinarias, Universidad Nacional de Rosario, Bv. Ovidio Lagos y Ruta 33, 2170 Casilda, Argentina; Centro Científico Tecnológico Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas - CONICET, Ocampo y Esmeralda, Rosario, Argentina.
Abstract:
The aim of this study was to determine the 96-h lethal concentration (96-h LC50) of lead (Pb) in silver catfish, Rhamdia quelen, and to determine histopathological biomarkers in fish exposed for 96-h to a sublethal concentration at 25% of the LC50. The 96-h LC50 was 108 mgl(-1). In gills, the length and thickness of lamella and thickness of the filament epithelium were significantly higher in fish exposed to Pb for 48-h than in control fish whereas the interlamellar distance decreased. In the liver, the area occupied by lipid droplets and size of hepatocytes showed significantly higher values after 24-h of exposure. The percentage of abnormal renal tubules was higher in fish exposed to Pb, exhibiting a time-dependent increase. These variations in histopathological biomarkers permit the definition of the overall response of R. quelen to Pb and the potential usefulness in the monitoring of Pb contamination.
