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Fusion of Secretory Vesicles with the Plasma Membrane

Fusion of Secretory Vesicles with the Plasma Membrane

Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...

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Related Experiment Video

Updated: Apr 19, 2026

Live Imaging of Synaptic Vesicle Recycling in the Neuromuscular Junction of Dissected Larval Zebrafish

Live Imaging of Synaptic Vesicle Recycling in the Neuromuscular Junction of Dissected Larval Zebrafish

Published on: February 7, 2025

A nanoscale resolution view on synaptic vesicle dynamics.

Dario Maschi¹, Vitaly A Klyachko

¹Department of Cell Biology and Physiology, Center for Investigations of Membrane Excitability Diseases, Washington University School of Medicine, St. Louis, Missouri; Department of Biomedical Engineering, Washington University, St. Louis, Missouri.

Synapse (New York, N.Y.)

|December 19, 2014

Summary

Efficient synaptic vesicle recycling is crucial for sustained neurotransmitter release. New super-resolution imaging techniques now allow visualization of vesicle cycling mechanisms within individual nerve terminals.

Keywords:

single particle tracking vesicle recycling

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Last Updated: Apr 19, 2026

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Published on: February 7, 2025

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Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons

Published on: September 4, 2017

Area of Science:

Neuroscience
Cell Biology

Background:

Synaptic function relies on efficient vesicle recycling for sustained neurotransmitter release.
Understanding vesicle recycling mechanisms is limited by the inaccessibility and small size of synaptic vesicles.
Conventional light microscopy cannot resolve the dynamics of synaptic vesicle cycling.

Purpose of the Study:

To summarize recent advances in studying synaptic vesicle recycling.
To highlight the application of nanoscale-resolution imaging techniques.
To reveal mechanisms controlling vesicle recycling in presynaptic terminals.

Main Methods:

Super-resolution imaging techniques
Single-particle tracking approaches
Analysis of dynamic processes within individual nerve terminals

Main Results:

Super-resolution microscopy overcomes diffraction limits for visualizing synaptic vesicles.
Single-particle tracking enables the study of dynamic vesicle movements.
New insights into the mechanisms controlling vesicle endocytosis and recycling are emerging.

Conclusions:

Nanoscale imaging provides unprecedented views of synaptic vesicle recycling.
These advanced techniques are crucial for understanding fundamental synaptic processes.
Further research using these methods will elucidate the precise control of neurotransmitter release.