Related Experiment Video
Updated: Apr 19, 2026

Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules
Published on: May 12, 2017
RNA recognition and stress granule formation by TIA proteins
Saboora Waris1, Matthew Charles James Wilce2, Jacqueline Anne Wilce3
1Department of Biochemistry and Molecular Biology, Monash University, Victoria 3800, Australia. saboora.waris@monash.edu.
Abstract:
Stress granule (SG) formation is a primary mechanism through which gene expression is rapidly modulated when the eukaryotic cell undergoes cellular stresses (including heat, oxidative, viral infection, starvation). In particular, the sequestration of specifically targeted translationally stalled mRNAs into SGs limits the expression of a subset of genes, but allows the expression of heatshock proteins that have a protective effect in the cell. The importance of SGs is seen in several disease states in which SG function is disrupted. Fundamental to SG formation are the T cell restricted intracellular antigen (TIA) proteins (TIA-1 and TIA-1 related protein (TIAR)), that both directly bind to target RNA and self-associate to seed the formation of SGs. Here a summary is provided of the current understanding of the way in which TIA proteins target specific mRNA, and how TIA self-association is triggered under conditions of cellular stress.
Related Concept Videos
Regulation of the Unfolded Protein Response
The Unfolded Protein Response
Other Stress Responses in Bacteria
Golgi Matrix Proteins
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
Bacterial Protein Maturation
Tail-anchoring of Proteins in the ER Membrane

